Here are instructions in case you run into trouble. They will help you avoid common mistakes, such as uploading a file with only one chain, files with odd naming conventions, or files that contain non-amino/nucleic acid groups at ATOM records.

Required input

Upload Instructions

Please register if you wish to maintain an archive of your KFC jobs. Users may also submit jobs using the login name guest, with or without an email address. If you do not provide an email address, your results will only be accessible via the job queue.

Place your mouse over the icon for information about each field.

Username (or type 'guest'):	AND	Email Address:
Upload Complex:	OR	PDB Code:
Protein 1 Chainlist:		Protein 2 Chainlist:
Job Name:
Note: You may leave the chain identifiers blank if your PDB file contains a complex of exactly two chains separated by a TER record. Do not upload structures containing only a single chain, as this will return an error.

Optional input

Consurf Scores:

Rosetta AlaScan:

Experimental Data:

Please do not submit PDB files containing only one chain! The KFC model requires a protein interface, meaning two or more chains in contact with one another. If you want to predict functional residues for a single protein chain, we highly recommend that you look into Consurf or Evolutionary Trace or one of the many websites mentioned in this paper.